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Molecular Dynamics Inc two-dimensional laser scanning densitometer
Two Dimensional Laser Scanning Densitometer, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two-dimensional+laser+scanning+densitometer/two+dimensional+laser+scanning+densitometer/pm26508620-65-13-16
Average 90 stars, based on 1 article reviews
two-dimensional laser scanning densitometer - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Pituitary adenylate cyclase-activating polypeptide acts synergistically with relaxin in modulating ovarian cell function in rats.
Article Snippet: Quantification of gelatinases was achieved by computerized image analysis using a two-dimensional laser scanning densitometer (Molecular Dynamics, Sunnyvale, CA, USA).

Article Title: Ovarian granulosa cells utilize scavenger receptor SR-BI to evade cellular cholesterol homeostatic control for steroid synthesis
Article Snippet: Signals on X-ray films were quantifiedusing a two-dimensional laser scanning densitometer (Molecular Dynamics, Sunnyvale,CA).

Quantitative Proteomics:

Article Title: Interplay of PI3K and cAMP/PKA signaling, and rapamycin-hypersensitivity in TGFβ1 enhancement of FSH-stimulated steroidogenesis in rat ovarian granulosa cells
Article Snippet: The primary antibody was visualized using horseradish peroxidase-conjugated antimouse or anti-rabbit IgG secondary antibodies and enhanced chemiluminescence (ECL; Amersham Pharmacia Biotech UK Limited). .. Relative quantification of ECL signals on X-ray film was analyzed using a two-dimensional laser scanning densitometer (Molecular Dynamics, Sunnyvale, CA, USA). ..

Article Title: Lindane, a gap junction blocker, suppresses FSH and transforming growth factor beta1-induced connexin43 gap junction formation and steroidogenesis in rat granulosa cells.
Article Snippet: Specific signals were detected using an enhanced chemiluminescence (ECL) detection system (Amersham Pharmacia Biotech UK Limited, Little Chalfont, Buckinghamshire, England) according to the manufacturer’s protocol. .. Relative quantification of ECL signals on x-ray film was analyzed using a two-dimensional laser scanning densitometer (Molecular Dynamics, Sunnyvale, CA, USA). ..

Article Title: CRTC2 and Nedd4 ligase involvement in FSH and TGFβ1 upregulation of connexin43 gap junction.
Article Snippet: At the end of culture, cell lysates were prepared in lysis buffer (radioimmunoprecipitation assay/RIPA buffer containing a protease inhibitor cocktail and 5 mM of phosphatase inhibitors, Na orthovanadate and NaF) by sonication using ultrasonicator (Missonix Models XL2020) and analyzed for Cx43 protein by immunoblotting using mouse monoclonal antibody (BD Transduction) as previously described (Ke et al. 2005). .. Relative quantification of chemiluminescent signals on x-ray film was analyzed using a two-dimensional laser scanning densitometer (Molecular Dynamics, Sunnyvale, CA, USA). .. In this study, gel electrophoresis was run for a longer time length than that in our earlier work (Ke et al. 2005), and we Published by Bioscientifica Ltd. Downloaded from Bioscientifica.com at 10/27/2023 06:42:41PM via free access 0.0 0.2 0.4 0.6 0.8 1.0 1.2 0.0 0.2 0.4 0.6 0.8 1.0 Vehicle PKAI C x4 3- P 1/ β- ac tin C x4 3- P 2/ β- ac tin Control FSH FSH + TGFβ1 Control FSH FSH + TGFβ1 Control FSH FSH + TGFβ1 a a b * a a b * a a b * a a b * * a a b * a a b * Vehicle SB431542 β-actin Cx43 P2 P1 PKAI SB C F FFT FT C F FFT FT C F FT FT CNI Vehicle CNI Figure 1 Effect of selective inhibitor of PKA (PKAI), calcineurin (CNI) and TGFbRI (SB431542) on the FSH and TGFb1-regulated Cx43 protein levels in rat ovarian granulosa cells.

Article Title: Potential role of follicle-stimulating hormone (FSH) and transforming growth factor (TGFβ1) in the regulation of ovarian angiogenesis.
Article Snippet: Specific signals were detected using a Western Lightning1 Plus–ECL, enhanced chemiluminescence substrate (PerkinElmer, Waltham, MA) according to the manufacturer’s protocol. .. Relative quantification of chemiluminescence signals on X-ray film was analyzed using a two-dimensional laser scanning densitometer (Molecular Dynamics, Sunnyvale, CA). ..

Article Title: Crucial role of estrogen receptor-alpha interaction with transcription coregulators in follicle-stimulating hormone and transforming growth factor beta1 up-regulation of steroidogenesis in rat ovarian granulosa cells.
Article Snippet: .. Relative quantification of chemiluminescent signals on x-ray film was analyzed using a two-dimensional laser scanning densitometer (Molecular Dynamics, Sunnyvale, CA). ..

Quantitation Assay:

Article Title: Critical involvement of ILK in TGFbeta1-stimulated invasion/migration of human ovarian cancer cells is associated with urokinase plasminogen activator system.
Article Snippet: .. Relative quantitation of ECL signal on X-ray film was analyzed using two-dimensional laser scanning densitometer (Molecular Dynamics, Sunnyvale, CA, USA). .. SKOV3 cells were cultured in 6-cm or 10-cm dish (Falcon Labware, Lincoln Park, NJ, USA), and treated with control or 10 ng/ml of TGFβ1 in serum-free medium for various time periods.



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